Dead Cas9 plasmid

半岛bd体育手机客户端 说明

一般描述

This product is an expression plasmid that utilizes the CMV promoter for strong transient expression of Dead Cas9 (CMV-dCas9). The dCas9 expression plasmid is one part of a two part CRISPR system with individual dCas9 and gRNA expression vectors.

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应用

Functional Genomics/Target Validation

• Proxy CRISPR
• Suitable for genomic DNA detection

特点和优势

• Highly specific
• Sequence verified
• Ready to use purified plasmid DNA

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacteriumStreptococcus pyogeneshas been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA).The Cas9 endonuclease can be rendered inactive (dCas9) with mutations to the two protein domains, RuvC and HnH (D10A and H840A respectively), which are responsible for nuclease activity. The nuclease deficient protein can then be programmed with a gRNA and directed to bind at a desired sequence of DNA. Variants of programmable endonucleases are often unable to cleave a large number of the targets that are efficiently cleaved by canonical SpCas9 in human cells. Cotargeting dead Cas9 may alter local chromatin structures and render otherwise inaccessible target sites now accessible and cleavable by alternative nucleases such as type II-B FnCas9, type II-C CjCas9 and NcCas9 and type V FnCpf1.

法律信息

CRISPR Label License

半岛bd体育手机客户端 性质

重组	expressed inE. coli
包装	vial of 50 μL
浓度	20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
启动子	Promoter name: CMV
application(s)	CRISPR
selection	kanamycin
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable