Olomoucine-CAS 101622-51-9-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A purine derivative that acts as a potent, reversible and selective inhibitor of p34^cdk1/cyclin B (IC₅₀= 7 ?M) and related kinases including p33^cdk2/cyclin A (IC₅₀= 7 ?M), p33^cdk2/cyclin E (IC₅₀= 7 ?M), p33^cdk5/p35 (IC₅₀= 3 ?M), and p44 MAP kinase (IC₅₀= 25 ?M). Acts by competing for the ATP binding domain of the kinase. Exhibits reduced sensitivity towards related kinases, p34^cdk4/cyclin D (IC₅₀>1 mM), and p40^cdk6/cyclin D3 (IC₅₀>250 ?M). Does not significantly affect the activity of other protein kinases at 1 mM. Known to inhibit DNA synthesis in interleukin-2-stimulated T lymphocytes and trigger G₁arrest similar to that observed in interleukin-2-deprived cells. Also used to synchronize cells in G₁. A 50 mM (5 mg/336 ?l) solution of Olomoucine (Cat. No. 495624) in DMSOis also available.
A purine derivative that acts as a potent, reversible, and selective inhibitor of p34^cdc2/cyclin B (IC₅₀= 7 ?M) and related kinases, including p33^cdk2/cyclin A (IC₅₀= 7 ?M), p33^cdk2/cyclin E (IC₅₀= 7 ?M), p33^cdk5/p35 (IC₅₀= 3 ?M), and p44^MAPK(IC₅₀= 25 ?M). Acts by competing for the ATP binding domain of the kinase. Exhibits reduced sensitivity towards related kinases, p34^cdk4/cyclin D1 (IC₅₀>1000 ?M) and p40^cdk6/cyclin D3 (IC₅₀>250 ?M). Does not significantly affect the activity of other protein kinases even at 1 mM concentrations. Known to inhibit DNA synthesis in interleukin-2 stimulated T lymphocytes and triggers G₁arrest similar to that observed in interleukin-2 deprived cells. Also used to synchronize cells in G₁.

包装

1, 5 mg in Plastic ampoule

生化/生理作用

Cell permeable: yes
Primary Target
p34^cdk1/cyclin B
Target IC₅₀: 7 ?M, 7 ?M, 7 ?M, 3 ?M, 25 ?M against p34cdk1/cyclin B, p33cdk2/cyclin A, p33cdk2/cyclin E, p33cdk5/p35, and p44 MAP kinase, respectively
Product competes with ATP.
Reversible: yes

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C.

其他说明

Abraham, R.T., et al. 1995.Biol. Cell83,105.
Mistelli, T., and Warren, G. 1995.J. Cell Sci.108,2715.
Glab, N., et al. 1994.FEBS Lett.353,207.
Vesel?, J., et al. 1994.Eur. J. Biochem.224,771.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C15H18N6O
分子量	298.34
MDL编号	MFCD00189360

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (TLC)
形式	crystalline solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
溶解性	DMSO: soluble
运输	ambient
储存温度	?20℃
InChI	1S/C15H18N6O/c1-21-10-18-12-13(17-9-11-5-3-2-4-6-11)19-15(16-7-8-22)20-14(12)21/h2-6,10,22H,7-9H2,1H3,(H2,16,17,19,20)
InChI key	GTVPOLSIJWJJNY-UHFFFAOYSA-N

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable