Anti-Vimentin antibody produced in goat

半岛bd体育手机客户端 说明

一般描述

The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

特异性

The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

免疫原

vimentin from cultured human foreskin fibroblasts.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis.
Anti-Vimentin antibody produced in goat has been used in following studies:

• immunohistochemistry
• western blotting
• immunocytochemistry
• flow cytometry

生化/生理作用

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.
Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号

MFCD00145922

半岛bd体育手机客户端 性质

质量水平	200
生物来源	goat
偶联物	unconjugated
抗体形式	whole antiserum
antibody product type	primary antibodies
克隆	polyclonal
分子量	antigen 58 kDa
包含	15 mM sodium azide
species reactivity	human, wide range
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunohistochemistry (frozen sections): suitable indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue western blot: 1:50 using HS-68 human foreskin cell extract
UniProt登记号	P08670
运输	dry ice
储存温度	?20℃
Gene Information	human ... VIM(7431)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)