半岛bd体育手机客户端 说明
一般描述
α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.
应用
α-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.
生化/生理作用
α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.
其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率;半乳糖基转移酶和α-乳清蛋白的复合物称为乳糖合成酶。将Asp87 或Asp88定点突变至Ala,可完全消除其对钙的强结合亲和力,并可将乳糖合成酶的刺激降低至最大速率的<3.5%。
重悬
溶于 1mL 水后为 0.9-1.4mg/mL
基本信息
EC 号 | 235-747-3 |
MDL编号 | MFCD00162025 |
NACRES | NA.61 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | bovine milk |
形式 | lyophilized powder |
分子量 | ~14.2 kDa |
technique(s) | microbiological culture: suitable |
UniProt登记号 | P00711 |
储存温度 | −20℃ |
Gene Information | cow ... LALBA(281894) |
Sigma-Aldrich