半岛bd体育手机客户端 说明

一般描述

Purine nucleoside phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme.

适用性

Suitable for the detection of Purine Nucleoside Phosphorylase activity in cell and tissue lysate or purified recombinant protein.

原理

In this Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed by the breakdown of inosine is further converted to uric acid using a developer. The uric acid is measured at a wavelength of 293 nm. Limit of quantification is 0.1μU recombinant purine nucleoside phosphorylase.

基本信息

NACRES

NA.25

半岛bd体育手机客户端 性质

detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H290 - H312 - H314 - H317 - H334 - H361d - H412
预防措施声明	P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable