半岛bd体育手机客户端 说明
一般描述
Phosphoglycerate Dehydrogenase (PHGDH) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3 phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer.
特点和优势
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit:
适用性
The kit is suitable for the measurement of PHGDH activity in various tissues (liver and kidney, etc.) and cells (adherent or suspension cells) and for the analysis of de novo L-serine biosynthesis.
原理
The Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, phosphoglycerate dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal at A450nm.
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| web metadata keyword.default | Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)|sigma-aldrich |
| detection method | colorimetric |
| 储存温度 | ?20℃ |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H314 - H335 |
| 预防措施声明 | P261 - P271 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 |
| 危险分类 | Eye Dam. 1 - Skin Corr. 1B - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
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