Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

免疫原

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer⁷⁹⁵) of human retinoblastoma protein conjugated to KLH.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• fluorescence-activated cell sorting (FACS) assays
• enzyme linked immunosorbent assay (ELISA)

生化/生理作用

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sitesin vitroby cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS⁷⁹⁵is required to inactivate Rb imposed growth suppression.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD03457570
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	RB-10, monoclonal
形式	buffered aqueous solution
分子量	antigen 110 kDa
species reactivity	bovine, hamster, mouse, rat, human
浓度	~2 mg/mL
technique(s)	immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization indirect ELISA: suitable microarray: suitable western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
同位素/亚型	IgG1
UniProt登记号	P06400
运输	dry ice
储存温度	?20℃
Gene Information	human ... RB1(5925) mouse ... Rb1(19645) rat ... Rb1(24708)