MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-)

半岛bd体育手机客户端 说明

一般描述

MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-) are pre-neoplastic mammary epithelial cells from a human adult female with ZFN modifications.
This cell line was derived from ATCC Catalog No. CRL-10317.

应用

Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:8
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12,Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum,Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin,Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin,Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor,Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone,Cat. No. H6909, to a final concentration of 0.5 ug/mL.
Cell freezing medium - DMSO 1X,Cat. No. C6164.

特点和优势

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) modifications on chromosomes 10q23.3 and 3q26.3

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human female mammary glands (Source Disease: Fibrocystic Disease)
OMIM登记号	171834 601728
储存温度	?196℃
Gene Information	human ... PIK3CA(5290), PTEN(5728)

组份列表

组份可单独销售

货号	组份
	MCF10A CONTROL CELLS 1 vial

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable