半岛bd体育手机客户端 说明

一般描述

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.

适用性

该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。

原理

The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λ_ex= 535/ λ_em= 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.

基本信息

EC 号	259-322-7
NACRES	NA.25

半岛bd体育手机客户端 性质

detection method	colorimetric fluorometric
储存温度	?20℃

组份列表

组份不可单独销售

货号	组份
	Collagenase (0.2%)
	Collagenase Stop Buffer
	Adipocyte Wash Buffer
	Adipocyte Lipolysis Buffer
	Glycerol Assay Buffer
	Glycerol Probe, in DMSO
	Glycerol Enzyme Mix
	Glycerol Standard, 100 mM
	Isoproterenol, 10 mM
	Cell Strainer

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H319 - H335
预防措施声明	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	10 - Combustible liquids
WGK	WGK 3