半岛bd体育手机客户端 说明
一般描述
Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and α-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, γ-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.
应用
Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture.
RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors.
RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate?in both toxicity and barrier integrity assays (( Z742750).
RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01).
特点和优势
Sigma′s human RPTEC control SA7K clone cells and knockout (KO) cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making them ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC KO cells
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation
其他说明
ADME/Tox cell lines license. These products are covered by the “Consumable Vial” License as described in Exhibit 1.
法律信息
These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.
OrganoPlate is a registered trademark of MIMETAS B.V.
基本信息
NACRES | NA.81 |
半岛bd体育手机客户端 性质
生物来源 | human female renal (proximal convoluted tubule epithelial cells) |
质量水平 | 200 |
形式 | liquid |
OMIM登记号 | 171050 |
储存温度 | ?196℃ |
Gene Information | human ... ABCB1(5243) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich