IA-MS Immunoaffinity Mass Spectrometry Kit-Streptavidin

半岛bd体育手机客户端 说明

一般描述

The Streptavidin IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enablingrobust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own biotinylated capture molecule and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injectionwith no SPE or other cleanup required.

应用

Target protein enrichment and quantification from complex background by mass spectrometry.

特点和优势

• Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
• 96-well plate format has potential for automation in a liquid-handling system
• Bead-free system circumvents messy clean-up and eliminates risk of column clogging
• Plate strips allow user to run as many or as few samples as desired

包装

Box with foam insert containing kit components.

组分

Streptavidin High Binding Capacity Coated PlateS2577-1EA
SOLu-TrypsinEMS0004-100UG
MS Denaturation SolutionEMS00010-10ML
Rapid Trypsin Digestion BufferEMS0009-30ML
Tris Buffered Saline with TWEEN^?20 (TBST)powder, pH 8.0T9039
Tris Buffered Saline (TBS) powder, pH 8.0T6664
EZ-Pierce^?plate sealZ721581-1EA

See datasheet for more information.

储存及稳定性

2 years when stored at 2-8 ℃.

Related Presentation

• Development of a Plate-based Immunoaffinity LC-MS/MS Workflow for Sensitive Quantitation of Free Infliximab and Adalimumab in Human Serum (Poster, PDF)

法律信息

EZ-Pierce is a trademark of Excel Scientific, Inc.
TWEEN is a registered trademark of Croda International PLC

半岛bd体育手机客户端 性质

形式	solid
适用性	suitable for mass spectrometry
运输	wet ice
储存温度	2-8℃

组份列表

组份不可单独销售

货号	组份
	Rapid Trypsin Digestion Buffer 30 mL
	MS Denaturation Solution

安全信息

象形图
警示用语：	Danger
危险声明	H226 - H301 + H311 + H331 - H370
预防措施声明	P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
危险分类	Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1
储存分类代码	3 - Flammable liquids
WGK	WGK 3
闪点(F)	75.2 °F
闪点(C)	24 ℃