Glycogen Phosphorylase Colorimetric Assay Kit

半岛bd体育手机客户端 说明

一般描述

Glycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.

特点和优势

Glycogen Phosphorylase Assay Kit:

• Detects as low as 10 mU in a variety of sample types.

适用性

The kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.

原理

The Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A₄₅₀nm. The A₄₅₀nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.

半岛bd体育手机客户端 性质

质量水平	100
web metadata keyword.default	Glycogen Phosphorylase Colorimetric Assay Kit|sigma-aldrich
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H314 - H412
预防措施声明	P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
储存分类代码	8A - Combustible, corrosive hazardous materials