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Duolink?PLA Multicolor Probemaker Kit-Far Red

品牌
Sigma-Aldrich
货号
DUO96040
规格纯度
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
价格
12296.7 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Duolink ?PLA Multicolor Probemaker Kit-Far Red
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

应用

Specificity
Far Red fluorescence detection reagents are often used with Cy?5 filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink?projects

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特点和优势

Multicolor Duolink?proximity ligation assay (PLA?) is an application of the Duolink?technologythat allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink?PLA Probemaker Guide for Multicolor Detection and the Duolink?PLA Multicolor Detection protocol to use this product.

  • Detect multiple PPI, PTM and low abundant protein events in a single sample.
  • Visualize protein complex formation and dissociation
  • Monitor protein signaling pathways
  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Detection of low abundant proteins due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Faster and more simple than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Components
Duolink ?PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink ?PLA probe.

This product is comprised of the following:
  • Far Red Oligo H DUO86040H
  • Far Red Oligo I DUO86040I
  • Conjugation Buffer DUO82033
  • Stop Reagent DUO82034
  • Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink ?PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink ?PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink ?PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink ?Wash Buffers and Mounting Medium.

法律信息

Cy is a registered trademark of Cytiva
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

technique(s) immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
荧光 λex646 nm; λem664 nm (Far Red)
λex646 nm; λem664 nm
适用性 suitable for fluorescence
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 10 - Combustible liquids

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