Duolink^?In Situ Orange Starter Kit Mouse/Goat

半岛bd体育手机客户端 说明

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^?In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

To perform a complete Duolink^?PLAin situexperiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink^?PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink^?PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^?In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^?PLAin situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^?projects

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特点和优势

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	100
半岛bd体育手机客户端 线	Duolink?
technique(s)	proximity ligation assay: suitable
荧光	λex554 nm; λem576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
适用性	suitable for fluorescence

组份列表

组份可单独销售

货号	组份
DUO92006	Duolink?In Situ PLA?Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
DUO92001	Duolink?In Situ PLA?Probe Anti-Mouse PLUS
DUO92007	Duolink?In Situ Detection Reagents Orange
DUO82049	Duolink?In Situ Wash Buffers, Fluorescence
DUO82040	Duolink?In SituMounting Medium with DAPI

安全信息

象形图
警示用语：	Warning
危险声明	H290 - H317 - H411
预防措施声明	P234 - P261 - P272 - P273 - P280 - P302 + P352
危险分类	Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable