Duolink^?In Situ Microplate Nuclear Stain, Anti-Fade

半岛bd体育手机客户端 说明

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Use the Multiwell Plates modifications to the Duolink^?In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

To perform a complete Duolink^?PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^?PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
Duolink^?In SituMicroplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink^?In Situ in microtiter plates. See the datasheet for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^?projects

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特点和优势

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

质量水平	200
半岛bd体育手机客户端 线	Duolink?
technique(s)	proximity ligation assay: suitable
荧光	λex360 nm; λem460 nm
适用性	suitable for fluorescence-detection automated sequencing suitable for microtiter plates
运输	dry ice
储存温度	?20℃

安全信息

象形图
警示用语：	Warning
危险声明	H317 - H411
预防措施声明	P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
危险分类	Aquatic Chronic 2 - Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3