Duolink^?In Situ Microplate Heat Transfer Block

半岛bd体育手机客户端 说明

一般描述

Duolink^?In SituMicroplate Heat Transfer Block is an aluminium block ideal for keeping an even temperature across the plate during the incubation steps when using DuolinkIn Situreagents in multiwell plates. Incubation in the pre-heated Heat Transfer Block increases staining efficiency and reproducibility between different plates, and reduces plate edge effects.

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^?In Situ Fluorescence Protocol and the Duolink^?In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

To perform a complete Duolink^?PLAin situexperiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^?PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Specificity
Duolink^?In SituMicroplate Heat Transfer Block will increase staining efficiency and reproducibility between different plates and reduce edge effects when using Duolink^?reagents in microtiter plates.

Keep the Heat Transfer Block preheated at 37℃ and keep it in the 37℃ incubator throughout the whole Duolink^?In Situprotocol. Place the microtiter plate in the Heat Transfer Block during incubation steps.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Linkage
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^?projects

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特点和优势

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

制备说明

Storage and Stability:When not in use, store the Heat Transfer Block at room temperature.

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

半岛bd体育手机客户端 线	Duolink?
technique(s)	proximity ligation assay: suitable
适用性	suitable for brightfield suitable for fluorescence
储存温度	20-25℃