DNAse I Activity Assay Kit (Fluorometric)

半岛bd体育手机客户端 说明

一般描述

Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity.

特点和优势

DNAse I Activity Assay Kit:

• The limit of quantification is 178 fmoles of DNA probe cleaved per minute per mL.

适用性

The kit is suitable for the Measurement of DNAse I activity of purified proteins, the quantitative analysis of DNAse I mutants and inhibitors, and the comparative examination of DNAse I activity in serum and other biological samples.

原理

The DNAse I Activity Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors, as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at λ_Ex= 651nm/λ_Em= 681nm. The limit of quantification (LOQ) is 178 fmoles of DNA probe cleaved per minute per mL.

半岛bd体育手机客户端 性质

质量水平	100
web metadata keyword.default	DNAse I Activity Assay Kit (Fluorometric)|sigma-aldrich
detection method	fluorometric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P284 - P304 + P340 + P312 - P501
危险分类	Resp. Sens. 1
储存分类代码	10 - Combustible liquids