Cholesterol/Cholesteryl Ester Kit II

半岛bd体育手机客户端 说明

一般描述

Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions.

特点和优势

Cholesterol/Cholesteryl Ester Kit II:

• Simple procedure with results in ～1 hour.

适用性

The kit is suitable for the quantification of cholesterol/cholesteryl ester in serum, cells and tissue samples.

原理

In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at A₄₅₀nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in the kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.

半岛bd体育手机客户端 性质

质量水平	100
web metadata keyword.default	Cholesterol/Cholesteryl Ester Kit II|sigma-aldrich
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H314
预防措施声明	P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
危险分类	Eye Dam. 1 - Skin Corr. 1B
储存分类代码	8A - Combustible, corrosive hazardous materials