Cathepsin L Activity Assay Kit

半岛bd体育手机客户端 说明

一般描述

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

特点和优势

Cathepsin L Activity Assay Kit:

• Simple procedure with results in 1-2 hours.

适用性

The kit is suitable for the measurement of cathepsin L in cell lysates.

原理

The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.

半岛bd体育手机客户端 性质

质量水平	100
web metadata keyword.default	Cathepsin L Activity Assay Kit|sigma-aldrich
detection method	fluorometric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H318 - H334
预防措施声明	P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类	Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
储存分类代码	10 - Combustible liquids