半岛bd体育手机客户端 说明

一般描述

Immunoglobulins have two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein which mainly helps in immune defense. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgG (Immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

特异性

Anti-Rat IgG (whole molecule) antibody recognizes rat (immunoglobulin G) IgG and is determined by immunoelectrophoresis (IEP) versus normal rat serum and rat IgG prior to conjugation.

免疫原

purified rat IgG

应用

Anti-Rat IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in western blotting and enzyme linked immunosorbent assay (ELISA).

生化/生理作用

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al.,Scand. J. Immunol.,8, Suppl. 7, 7 (1978).

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号

MFCD00162813

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	peroxidase conjugate
抗体形式	IgG fraction of antiserum
antibody product type	secondary antibodies
克隆	polyclonal
形式	buffered aqueous solution
species reactivity	rat
technique(s)	direct ELISA: 1:40,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody
运输	dry ice
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H317 - H334
预防措施声明	P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
危险分类	Resp. Sens. 1 - Skin Sens. 1
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable