半岛bd体育手机客户端 说明

一般描述

Protein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.
Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
PGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.

免疫原

Mouse
a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α

包装

1 set in Plastic ampoule

应用

Immunoblotting (1 ?g/ml)

Immunocytochemistry (5-10 ?g/ml)

Immunoprecipitation (10 ?g/ml)

Paraffin Sections (whole mount) (see application references)

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

外形

One vial of 100 ?g antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 ?l whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 ?l per lane; add sample buffer prior to SDS-PAGE loading.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Endogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
Lai, L., et al. 2008.Genes Dev.14,1948.
Rodgers, J.T., et al. 2008.FEBS Lett.582,46.
Mazzucotelli, A., et al. 2007.Diabetes10,2467.
Nemoto, S., et al. 2005.J. Biol. Chem.16,16456.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	4C1.3, monoclonal
形式	liquid
不包含	preservative
species reactivity	human, rat, mouse
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG
储存温度	?70℃

安全信息

储存分类代码

10 - Combustible liquids