SW48 Cells PTEN -/-

半岛bd体育手机客户端 说明

一般描述

SW48 Cells PTEN -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

应用

Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM,Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum,Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate,Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine,Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.

特点和优势

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 10q23.3

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human female colon (Source Disease: Colorectal adenocarcinoma)
OMIM登记号	601728
储存温度	?196℃
Gene Information	human ... PTEN(5728)

组份列表

组份可单独销售

货号	组份
	SW48 Cells PTEN -/- 1 vial
	SW48 Control Cells 1 vial