SW48 Cells HIF1A -/-/-

半岛bd体育手机客户端 说明

一般描述

SW48 Cells HIF1A -/-/- are colorectal adenocarinoma, epithelial cells, from a human adult female, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-231.

应用

Media Renenewal changes one to two times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:2 to 1:6.
The base medium for this cell line is DMEM,Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum,Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate,Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X,Cat. No. C6164.

特点和优势

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human female colon (Source disease: Colorectal adenocarcinoma)
储存温度	?196℃
Gene Information	human ... HIF1A(3091)

组份列表

组份可单独销售

货号	组份
	SW48 CELLS HIF1A -/-/- 1 vial