半岛bd体育手机客户端 说明
一般描述
Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In the presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in the central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as part of the body′s defense mechanism.
特点和优势
Nitric Oxide Synthase (NOS) Activity Assay Kit:
适用性
The kit is suitable for the detection of NOS activity in purified recombinant protein and tissue or cell extracts.
原理
In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| web metadata keyword.default | Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)|sigma-aldrich |
| detection method | colorimetric |
| 储存温度 | ?70℃ |
安全信息
| 象形图 |      |
| 警示用语： | Danger |
| 危险声明 | H272 - H290 - H301 - H315 - H319 - H341 - H351 - H361d - H373 - H400 |
| 预防措施声明 | P202 - P210 - P273 - P301 + P310 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 3 Oral - Aquatic Acute 1 - Carc. 2 - Eye Irrit. 2 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 3 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 |
| 靶器官 | Nervous system |
| 储存分类代码 | 5.1B - Oxidizing hazardous materials |
| WGK | WGK 3 |
Sigma-Aldrich
m.cnreagent.com
