半岛bd体育手机客户端 说明
一般描述
The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
应用
特点和优势
原理
In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.
法律信息
LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES
半岛bd体育手机客户端 性质
包装 | pkg of 5 vials (5x200?L aliquots ) |
浓度 | ≥5x108 VP/ml (via p24 Assay) |
application(s) | CRISPR |
运输 | dry ice |
储存温度 | ?70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich