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Mouse CRISPR Activation Caprano Library

品牌
Sigma-Aldrich
货号
PCRISPR002
包装型号
规格纯度
价格
177930 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Mouse CRISPR Activation Caprano Library
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

  • Functional Genomics/Target Validation
  • Focused forward genetic activation screening
  • Validated positive and negative controls

特点和优势

  • Focus on your research, and we will generate your lentivirus screening library
  • Compatible with SAMHELPERV for consistent effector expression across a wide variety of cell lines dCas9-VP64-Blasticidin MS2-P65-HSF1-Hygromycin
  • Targeting Genes22,774 (Set A), 22,658 (Set B)
  • Compact Libary with gRNAs 67,187 (Set A), 66,889 (Set B)
  • Built-in Controls 500, unique non-targeting controls are in each of the two half-libraries (Set A and Set B)

组分

2 Subpools with a minimum concentration of 5x108VP/mL(via p24 assay)
PCRISPR002A - Mouse CRISPR Activation Caprano Library Set A
PCRISPR002B - Mouse CRISPR Activation Caprano Library Set B

原理

The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation.
CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number.

法律信息

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

半岛bd体育手机客户端 性质

包装 pkg of 10 vials (5x200?L aliquots of each component)
浓度 ≥5x108 VP/ml (via p24 Assay)
application(s) CRISPR
运输 dry ice
储存温度 ?70℃

安全信息

象形图 GHS07
警示用语: Warning
危险声明 H317
预防措施声明 P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类 Skin Sens. 1
储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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