MISSION^?LentiPlex^?Human Pooled shRNA Library TRC2

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一般描述

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Human shRNA Pooled Library 2.0 includes additional gene and clone coverage from the previously offered LentiPlex 1.0 Pooled Library . With the addition of 2.0, which consists of 5,000+ human genes and 20,000+ total clone coverage divided into 3 subpools, the complete whole-genome human librarycomplete whole-genome human librarynow consists of over 125,000 shRNA constructs from the TRC collection targeting 20,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The complete library is provided in ready-to-use lentiviral format at titers of at least 5 x 10⁸TU/ml via p24 assay and is pre-divided into sixteen subpools of approximately 6,000-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.

其他说明

The MISSION LentiPlex Human Pooled shRNA Libraries consist of Catalog Numbers SHPH01, SHPH15, SHPH2, and SHPHLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library.

Amplification and sequencing primers are also provided for downstream hit identification.

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

• Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
• Comprehensive, reproducible results from pooled shRNA screens
• Statistically robust and information-rich data