半岛bd体育手机客户端 说明
一般描述
MCF10A CELLS HER2 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.
应用
Cell freezing medium-DMSO 1X,Cat. No. C6164
Media renewal changes two to three times per week.
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12,Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum,Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin,Cat No. C8052, to a final concentration of 1 ng/mL; human insulin,Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor,Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone,Cat No. H6909, to a final concentration of 0.5 ug/mL.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
生化/生理作用
Human epidermal growth factor receptor 2 (HER2) signaling pathway stimulates cell proliferation and survival in the majority of breast cancers. Thus, overexpression of this protein causes breast cancer. Heterodimeric complex of HER2 and phosphatidylinositide 3-kinase (PI3K) is the most potent stimulator of the PI3K/AKT anti-apoptosis pathway. Increased expression of HER2 is also observed in ovarian, colorectal, pancreatic, endometrial and gastric cancers. Trastuzumab, an antibody, works by binding to a domain in the external domain of HER2. This domain is missing in p95, a truncated form of HER2, and hence these cancer cells show resistance to trastuzumab. HER2 protein can be used as a prognostic marker and as a therapeutic option for gynecologic cancers.
特点和优势
Zinc finger nuclease (ZFN) knockout on chromosome 17q21.1
These MCF10A cells are adherent with a doubling time of approx. 20 hours
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
基本信息
eCl@ss | 32190102 |
NACRES | NA.81 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | human female mammary glands (Source Disease: Fibrocystic Disease) |
OMIM登记号 | 164870 |
储存温度 | ?196℃ |
Gene Information | human ... ERBB2(2064), HER2(2064) |
组份列表
组份可单独销售
货号 | 组份 |
MCF10A CONTROL CELLS 1 vial | |
MCF10A CELLS HER2 (-/-) 1 vial |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich