半岛bd体育手机客户端 说明
一般描述
Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. Of the several classes of HDACs, this kit assays a member of the zinc-independent, NADH requiring class, HDAC3. With Sigma′s HDAC3 Inhibitor Screening Kit, HDAC3 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC3 Substrate [Arg-His-Lys-Lys(Ac)-AFC]. This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC3 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.
特点和优势
其他说明
The kit is shipped on dry ice.Store HDAC-3 enzyme at -80 ℃ upon receipt.All other components should be stored at -20 ℃, protected from light. All -20 ℃ reagents should be used within 2 months after thawing.
基本信息
| 酶学委员会(EC)编号 | 3.5.1.98 ( BRENDA | IUBMB ) |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 用途 | 100 assays in 96 well plates |
| NCBI登记号 | NM_003883 (Human) NP_034541 (Mouse) |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... HDAC3(8841) mouse ... HDAC3(15183) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| 闪点(F) | 188.6 °F - closed cup |
| 闪点(C) | 87 ℃ - closed cup |
Sigma-Aldrich
m.cnreagent.com
