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位置: 首页> 品牌> Sigma-Aldrich> Homocysteine Assay Kit

Homocysteine Assay Kit

品牌
Sigma-Aldrich
货号
MAK354
规格纯度
sufficient for 100 fluorometric tests
价格
5832.91 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Homocysteine Assay Kit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine (‘free′ homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke.

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for the determination of total homocysteine concentration in plasma or serum.

原理

The Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (λex = 658 nm/λem = 708 nm). The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 μM homocysteine.

半岛bd体育手机客户端 性质

用途 sufficient for 100 fluorometric tests
detection method fluorometric

安全信息

象形图 GHS05
警示用语: Warning
危险声明 H290 - H315 - H319 - H412
预防措施声明 P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类 Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
储存分类代码 8A - Combustible, corrosive hazardous materials
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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