半岛bd体育手机客户端 说明
应用
Specificity
Green fluorescence detection reagents are often used with FITC filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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特点和优势
Multicolor Duolink?proximity ligation assay (PLA?) is an application of the Duolink?technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink?PLA Probemaker Guide for Multicolor Detection and the Duolink?PLA Multicolor Detection protocol to use this product.
组分
Duolink?PLA Multicolor Probemaker Kit - Green contains all the necessary reagents to perform the conjugations of Green Oligo C and GreenOligo D directly to primary antibodies. The resulting PLA probes (Green C and Green D) must be used together and cannot be paired with any other Duolink?PLA Probe.
This product is comprised of the following:
法律信息
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC
半岛bd体育手机客户端 性质
半岛bd体育手机客户端 线 | Duolink? |
technique(s) | immunofluorescence: suitable multiplexing: suitable proximity ligation assay: suitable |
荧光 | λex490 nm; λem520 nm (FITC) λex490 nm; λem520 nm (green) |
适用性 | suitable for fluorescence |
运输 | dry ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |