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位置: 首页> 品牌> Sigma-Aldrich> Duolink?PLA Multicolor Probemaker Kit-Orange

Duolink?PLA Multicolor Probemaker Kit-Orange

品牌
Sigma-Aldrich
货号
DUO96030
规格纯度
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
价格
12296.7 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Duolink ?PLA Multicolor Probemaker Kit-Orange
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

应用

Specificity
Orange fluorescence detection reagents are often used with Cy?3 filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink?projects

View full Duolink?product list

特点和优势

Multicolor Duolink?proximity ligation assay (PLA?) is an application of the Duolink?technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink?PLA Probemaker Guide for Multicolor Detection and the Duolink?PLA Multicolor Detection protocol to use this product.

  • Visualize and detect multiple proteins events in one experiment.
  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Detection of low abundant proteins due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Faster and more simple than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Components
Duolink ?PLA Multicolor Probemaker Kit - Orange contains all the necessary reagents to perform the conjugations of Orange Oligo F and Orange Oligo G directly to primary antibodies. The resulting PLA probes (Orange F and Orange G) must be used together and cannot be paired with any other Duolink ?PLA probe.

This product is comprised of the following:
  • Orange Oligo F DUO86030F
  • Orange Oligo G DUO86030G
  • Conjugation Buffer DUO82033
  • Stop Reagent DUO82034
  • Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink ?PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink ?PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink ?PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink ?Wash Buffers and Mounting Medium.

法律信息

Cy is a registered trademark of Cytiva
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

半岛bd体育手机客户端 线 Duolink?
technique(s) immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
荧光 λex542 nm; λem562 nm (orange)
λex542 nm; λem562 nm
适用性 suitable for fluorescence
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 10 - Combustible liquids

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