DLD1 Cells SMAD4 -/-

半岛bd体育手机客户端 说明

一般描述

DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.

应用

Media Renenewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI,Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum,Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate,Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X,Cat. No. C6164.

特点和优势

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

生物来源	human male colon (Source Disease: Colorectal adenocarcinoma)
质量水平	200
OMIM登记号	600993
储存温度	?196℃
Gene Information	human ... SMAD4(4089)

组份列表

组份可单独销售

货号	组份
	DLD1 Control Cells