DLD1 Cells HIF1A -/-

半岛bd体育手机客户端 说明

一般描述

This product corresponds to ATCC Cat. No.CCL-221
DLD1 Cells HIF1A -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

应用

Media Renewal changes two to three times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is RPMI,Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum,Cat. No. F4135, to a final concentration (v/v) of 10%.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:10

特点和优势

Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

生物来源	human male colon (Source Disease: Colorectal adenocarcinoma)
质量水平	200
OMIM登记号	603348
储存温度	?196℃
Gene Information	human ... HIF1A(3091)

组份列表

组份可单独销售

货号	组份
	DLD1 Control Cells 1 vial
	DLD1 Cells HIF1A -/- 1 vial