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位置: 首页> 品牌> Sigma-Aldrich> DLD1 CELLS AKT2 -/-

DLD1 CELLS AKT2 -/-

品牌
Sigma-Aldrich
货号
CLLS1133
包装型号
规格纯度
价格
59056.1 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
DLD1 CELLS AKT2 -/-
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.

应用

Media Renenewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI,Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum,Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine,Cat. No. G7513, to a final concentration of 2 mM and sodium pyruvate,Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X,Cat. No. C6164.

特点和优势

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 19q13.1-q13.2

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRES NA.81

半岛bd体育手机客户端 性质

生物来源 human male colon (Source Disease: Colorectal adenocarcinoma)
质量水平 200
OMIM登记号 164731
储存温度 ?196℃
Gene Information human ... AKT2(208)

组份列表

组份可单独销售

货号 组份
DLD1 Control Cells
DLD1 CELLS AKT2 -/-

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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