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位置: 首页> 品牌> Sigma-Aldrich> D-Lactate Assay Kit

D-Lactate Assay Kit

品牌
Sigma-Aldrich
货号
MAK336
规格纯度
sufficient for 100 colorimetric tests
价格
4962.3 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
D-Lactate Assay Kit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for D-lactate in serum, plasma, and cell media samples.

原理

The D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

半岛bd体育手机客户端 性质

用途 sufficient for 100 colorimetric tests
detection method colorimetric
储存温度 ?20℃

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2

Sigma-Aldrich

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