半岛bd体育手机客户端 说明
一般描述
Near-IR CF dyes are a group of fluorescent dyes with absorption and emission wavelengths between 650 and 800 nm. Near-IR CF dyes are significantly brighter and more stable than any other commercial dye of similar wavelengths. Near-IR CF dyes offer important advantages over traditional visible light dyes. Because cellular or tissue components produce minimal autofluorescence in the near-IR region, near-IR dyes have the potential to offer highly specific and sensitive detection in complex biological systems. Also, because light with wavelength in the near-IR region has strong tissue penetration, near-IR dyes are ideal for in vivo fluorescence imaging, an emerging field that has advanced rapidly in recent years. Futhermore, near-IR dyes are also excellent dyes for in- or on-cell and membrane-based Western assays. CF750 is so bright, it can be excited at 633 nm, but still emits stronger fluorescence at ~770 nm than APC-based tandem dyes, making the dye particularly useful for flow cytometry applications without the spillover and stability challenges encountered with tandem dyes.
CF?750 succinimidyl ester (CF750 SE) is used for labeling proteins or other biomolecules having an amine group. The succinimidyl ester group of the dye reacts with an amine group to form a stable amide linkage.
应用
CF?750 succinimidyl ester is suitable for labelling antibodies during for xenograft Imaging of CD25-positive tumors. It may also be used for labelling proteins to track the protein delivery in nasal mucosa using nanoparticles containing an anionic lipid in their core (NPL).
特点和优势
1. Near-IR CF dyes are exceptionally bright and stable. 2. Water-soluble. 3. Ideal forin vivoimage. 4. Less immunogenic than other competitive dyes.
制备说明
Protect from light and moisture
重悬
Dissolve in DMSO
法律信息
This product is distributed by sigma-aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
测定 | ≥90% (TLC) |
形式 | solid |
分子量 | ~3,009 g/mol (free acid) |
溶解性 | DMF: soluble DMSO: soluble H2O: soluble |
ε (extinction coefficient) | 250000 at 755 nm |
吸光度比值 | A280/755 nm0.03 |
荧光 | λex680 or 685 nm (Flow cytometry laser line) λex680 or 685 nm (Microscopy laser line) λex755 nm; λem777 nm |
适用性 | suitable for fluorescence |
运输 | wet ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich