半岛bd体育手机客户端 说明
一般描述
Immunoglobulin M (IgM) belongs to the immunoglobulin family. It exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.
特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Alkaline Phosphatase (calf intestine), Anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat or Human heavy or light chain isotypes.
免疫原
Rat IgM mu heavy chain
物理属性
Antibody format: IgG
外形
Supplied in 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.46 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | goat |
| 偶联物 | alkaline phosphatase conjugate |
| 抗体形式 | affinity isolated antibody |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rat |
| 浓度 | 1.0 mg/mL |
| technique(s) | immunohistochemistry: suitable indirect ELISA: suitable western blot: suitable |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
