Aconitase Assay Kit

半岛bd体育手机客户端 说明

一般描述

Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe⁴S⁴] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich′s ataxia and diabetes.

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

原理

The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

半岛bd体育手机客户端 性质

用途	sufficient for 100 colorimetric tests
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P284 - P501
危险分类	Resp. Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3