5′-Nucleotidase (CD73) Activity Assay Kit (Colorimetric)

半岛bd体育手机客户端 说明

一般描述

5′-Nucleotidase (5′-NT), also known at CD73 is an enzyme located in the plasma membrane. It converts extracellular nucleotides like 5′-AMP to their corresponding nucleosides, through phosphorylitic cleavage. This conversion facilitates uptake of the nucleosides through nucleoside receptors into the cell, where they can again be phosphorylated to generate nucleotides and contribute to the nucleotide pool, inside the cell. 5′-NT levels are elevated in hepatic diseases such as viral hepatitis, alcoholic liver disease and cirrhosis.

特点和优势

5′-Nucleotidase (CD73) Activity Assay Kit:

• Sensitive, detecting as low as 0.2 mU of 5′-NT.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the measurement of 5′-Nucleotidase activity in animal tissue lysates (eg. liver), cell lysates, recombinant enzyme and purified protein.

原理

The 5′-Nucleotidase (CD73) Activity Assay Kit is a simple two-step end point assay that relies on the Berthelot′s test for quantification of ammonia. In this assay, the action of 5′-nucleotidase on the substrate generates a product, which releases ammonia in presence of the converter. Developer I and II are then used to quantify the released ammonia through increase in absorbance at A₆₇₀nm. This assay can detect as low as 0.2 mU of 5′-NT. Since non-specific enzymes like alkaline phosphatase can give a positive signal in this assay, 5′-NT inhibitor may be used to completely inhibit 5′-nucleotidase and distinguish from the signal from non-specific enzymes. The assay kit also includes 5′- Nucleotidase (5′-NT) enzyme for use as a positive control.

半岛bd体育手机客户端 性质

质量水平	100
web metadata keyword.default	5′-Nucleotidase (CD73) Activity Assay Kit (Colorimetric)|sigma-aldrich
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H317 - H318 - H334 - H341 - H350i - H360D - H372 - H373 - H411
预防措施声明	P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
危险分类	Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
靶器官	Lungs
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects