Monoclonal Anti-v-Src antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.

免疫原

Src protein (p60^src).

应用

The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.

外形

Lyophilized with 100 μg bovine serum albumin.

分析说明

p60^srcmay appear as a doublet due to phosphorylation.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00239655
NACRES	NA.44

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	327, monoclonal
形式	lyophilized powder
分子量	antigen ~56 kDa
species reactivity	mouse, human, rat
technique(s)	immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation) western blot: 2-5 μg/mL using COLO201 cell extract
同位素/亚型	IgG1κ
UniProt登记号	P12931
运输	wet ice
储存温度	2-8℃
Gene Information	human ... SRC(6714) mouse ... Src(20779) rat ... Src(83805)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)