半岛bd体育手机客户端 说明

应用

Complement C3 is important in complement activation through the classical and alternative pathways. Complement C3 has been used in studies of bacterial defense against the complement system. Research on streptococcus infection has shown thatStreptococcal pyrogenicexotoxin B (SPE B), a cysteine protease, can prevent phagocytic activity via cleavage of the C3 and impairing the pathways of the compliment system. C3 deficiency can result in recurrent infections and immune complex disorders. Recent research has shown that inherited C3 deficiency can occur either through homozygous mutations or through compound heterozygous mutations,

生化/生理作用

Complement C3 is the third and most abundant component of the complement pathway. It plays a central role in complement activation, being involved in both the classical and alternative pathways. C3 or its proteolytic fragments mediate many biological functions such as opsonization and anaphylatoxin activities. Pro-C3 is synthesized as a large single chain of 185 kDa and is processed to a disulfide-linked heterodimer consisting of an α-chain (115 kDa) and a β-chain (70 kDa) in the plasma. C3 is cleaved by the C3 convertases in either pathway (C4b2a, C3iBb, C3bBb) to C3b (176 kDa) comprised of the large C-terminal portion of the α-chain and the β-chain. C3b interacts with other complement components to initiate the amplification cascade. Studies of C3 function have been facilitated by experimentally induced C3 deficiency in animals by injection of cobra venom factor and more recently with gene ablation technology.

质量

Functionally pure by a hemolytic assay using deficient sera.

外形

Supplied as a solution in PBS, pH 7.2.

其他说明

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

基本信息

MDL编号	MFCD00130836
NACRES	NA.61

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
测定	≥85% (SDS-GE)
形式	solution
specific activity	≥3000 C3H50 units/mg (using C3 deficient serum)
分子量	185 kDa (disulfide-linked heterodimer)
technique(s)	activity assay: suitable
UniProt登记号	P01024
运输	dry ice
储存温度	?70℃
Gene Information	human ... C3(718)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable