Cathepsin D/E Substrate, Fluorogenic-Calbiochem

半岛bd体育手机客户端 说明

一般描述

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m= 15.6 ?M^-1s^-1and 10.9 ?M^-1s^-1for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m= 15.6 ?M^-1s^-1and 10.9 ?M^-1s^-1for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.

包装

1 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Substrate for cathepsins D and E
kcat/Km = 15.6 ?M?? sec?? and 10.9 ?M?? sec?? for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively

警告

Toxicity: Standard Handling (A)

序列

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH?

MOCAc = (7-Methoxycoumarin-4-yl)acetyl

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Yasuda, Y., et al. 1999.J. Biochem.126,260.
Yasuda, Y., et al. 1999.J. Biochem.125,1137.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C85H122N22O19
分子量	1756.01

半岛bd体育手机客户端 性质

质量水平	100
测定	≥97% (HPLC)
形式	lyophilized solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze desiccated (hygroscopic) protect from light
颜色	yellow
溶解性	DMSO: 5 mg/mL
荧光	λex~328 nm λem~393 nm
运输	ambient
储存温度	2-8℃