Anti-Serine/Threonine Protein Phosphatase 2 A/Bα antibody produced in rabbit

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一般描述

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein phosphatase 2A (PP2A) holozyme consists of a catalytic subunit (C), a structural subunit (A) and a regulatory subunit (B). The subunit B dictates the substrate specificity and is coded by at least 13 genes resulting in 3 distinct classes of subunits - α, β and γ. The B α subunit is targeted to the microtubules, renders substrate specificty and dephosphorylates cyclin-dependent kinase sites. The PP2A holoenzyme has been implicated in various functions such as cell cycle progression, oncogenic transformation and is subjected to tight regulation at the translational level, by post translational modifications and by protein-protein interations.
Anti-Serine/Threonine Protein Phosphatase 2A/Bα recognizes protein phosphatase 2A/Bα?isoforms of 55 kDa.

免疫原

synthetic peptide (FSQVKGAVDDDVAE) corresponding to the N-terminus (amino acids 14-27) of the protein phosphatase 2A/Bα subunit.

应用

Anti-Serine/Threonine Protein Phosphatase 2A/B α may be used for detection at working concentration of 5-10 μg/mL in total rat brain homogenate. The antibody is also suitable for immunocytochemistry at a working concentration of 5-10 μg/mL.

外形

Provided as a 0.2 μm filtered solution in phosphate buffered saline containing 0.08% sodium azide.

基本信息

MDL编号	MFCD03455946
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 55 kDa
species reactivity	human, bovine, rat, mouse
浓度	~1 mg/mL
technique(s)	immunocytochemistry: 5-10 μg/mL western blot: 5-10 μg/mL using total rat brain homogenate
UniProt登记号	P63151
运输	dry ice
储存温度	?20℃
Gene Information	human ... PPP2R2A(5520)