Anti-phospho-DARPP32 (pThr⁷⁵) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.

免疫原

synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.

应用

Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.

生化/生理作用

Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac^?.

目标描述

DARPP32 phosphorylated on Thr⁷⁵acts as an inhibitor of PKA.

外形

Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

其他说明

The labeling by the antibody to DARPP32 Thr⁷⁵is markedly increased by okadaic acid treatment.

法律信息

Prozac is a registered trademark of Eli Lilly and Co.

基本信息

MDL编号	MFCD08705496
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
用途	sufficient for 10 blots
分子量	antigen 32 kDa
species reactivity	mouse, human
technique(s)	dot blot: 1:1,000 western blot: 1:1,000
UniProt登记号	Q9UD71
运输	wet ice
储存温度	?20℃
Gene Information	human ... PPP1R1B(84152) mouse ... Ppp1r1b(19049)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)