QuickLink^TMDNA Ligation Kit

半岛bd体育手机客户端 说明

一般描述

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.

应用

QuickLink^?DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2
The QuickLink^?DNA Ligation Kit is suitable for:

• Joining blunt or cohesive-end fragments of DNA into a cloning vector
• Recircularization of linear DNA
• Formation of concatamers
• dsDNA nick repair

特点和优势

• Fast 5 minutes ligation
• High ligation efficiency
• Room temperature reactions – no cooling required
• Perform bacterial transformation with the reaction mixture

组分

Sufficient for 50 reactions:

• 500uL 2X Ligation Buffer A (L9537)
• 100uL 5X Ligation Buffer B (L9662)
• 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT

原理

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

法律信息

QuickLink is a trademark of sigma-aldrich Co. LLC

基本信息

EC 号

232-770-0

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
形式	liquid
用途	1 kit sufficient for 50 ligation reactions
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable