Chaperonin 60 fromEscherichia coli

半岛bd体育手机客户端 说明

应用

Chaperonin 60 fromEscherichia colihas been used:

• in mass spectroscopy
• in cryo-electron microscopy imaging as control for testing particle distribution
• as standard in infrared spectrum measurements

包装

1 mg in serum bottle
Package size based on protein content.

生化/生理作用

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides.In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

外形

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

基本信息

MDL编号	MFCD00163256
NACRES	NA.32

半岛bd体育手机客户端 性质

生物来源	Escherichia coli
质量水平	200
重组	expressed inE. colioverproducing strain
测定	>95% (SDS-PAGE)
形式	lyophilized powder
technique(s)	electron microscopy: suitable mass spectrometry (MS): suitable
UniProt登记号	P10809
储存温度	2-8℃
Gene Information	human ... HSPD1(3329)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)