Anti-Platelet-Derived Growth Factor antibody produced in goat

半岛bd体育手机客户端 说明

一般描述

Platelet-derived growth factor (PDGF) is a serum growth factor purified from human platelets. The members of PDGF are homodimers of A-, B-, C- and –D polypeptide chains and an AB heterodimer. PDGF is a mitogen and stimulates the proliferation of cells of connective tissue and glial cells, survival, differentiation, chemotaxis and wound healing. The cellular effects of PDGF isoforms are mediated by binding to α- and β-receptors which leads to activation of downstream signaling via PI3K, STAT and Ras/MAPK. Overexpression of PDGF isoforms has been observed in various malignancies including gliomas, osetosarcoma, chronic myelomonocytic leukemia and non-small-cell lung cancers
Anti-Human Platelet-Derived Growth Factor (PDGF) specifically recognizes natural human PDGF. It will also neutralize the activity of recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. It shows cross reactivity with recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB.

特异性

The antibody will neutralize the biological activity of natural human PDGF, recombinant human PDGF-AB, PDGF-AA and PDGF-BB and natural porcine PDGF-BB.

免疫原

natural human PDGF.

应用

Anti-Human Platelet-Derived Growth Factor (PDGF) antibody may be used for ELISA at a working concentration of 0.25 μg/ml. The antibody has been used for ELISA at a dilution of 1:500 using human serum.. For immunoblotting, a concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions and has been used to neutralize PDGF of primary Human dermal fibroblasts (HDFs) and human platelet-enriched plasma.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) with 5% trehalose.

制备说明

Purified using protein G.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00165456
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	goat
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	lyophilized powder
species reactivity	human
technique(s)	indirect ELISA: suitable neutralization: suitable western blot: suitable
UniProt登记号	P01127 P04085
储存温度	?20℃
Gene Information	human ... PDGFA(5154), PDGFB(5155)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)