Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr²²³) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

SAPK/ERK-1 kinase (SEK1) also called MKK4 or Jun kinase kinase (JNKK) activates the stress activated protein kinase (SAPK). There are other mechanisms by which SAPK may be activated but SEK1 being the immediate upstream activator defines the ensuing protein kinase cascade by the c-Jun transcription factor. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at position 219 and 223 respectively by MEKK. High levels of JNK/SAPK activity are reported to induce apoptosis by the activation of c-Jun. Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK1, JNKK1 (phosphothreonine 223) detects SEK1/MKK4 protein when activated by phosphorylation at Thr223.

特异性

Does not react with non-phosphorylated MKK4/SEK1 or phosphorylated MKK3, MEK-1 or MEK-2.

免疫原

synthetic phospho-Thr²²³peptide corresponding to residues around Thr²²³of human SEK1.

应用

Anti-phospho-MAP Kinase Kinase 4 may be used for immunoblotting at a working dilution of 1:2,000 using sodium chloride and vanadate-treated 293 cells.

外形

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin and 50% glycerol.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02097162
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
species reactivity	rat, mouse, human
technique(s)	western blot (chemiluminescent): 1:2,000 using sodium chloride and vanadate-treated 293 cells
UniProt登记号	P45985
运输	wet ice
储存温度	?20℃
Gene Information	human ... MAP2K4(6416) mouse ... Map2k4(26398)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable