Anti-phospho-MAP Kinase Kinase 1/2 (pSer^217/221) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells.
The antibody specifically recognizes MEK1/2 (45 kDa) only when phosphorylated at Ser²¹⁷and Ser²²¹. It reacts strongly with MEK1/2 phosphorylated only at Ser²¹⁷, but less when phosphorylated only at Ser²²¹. It shows not cross reaction with any other related family members including SEK.

特异性

Detects MEK 1/2 when phosphorylated at Ser²¹⁷and Ser²²¹. It does not react with non-phosphorylated MEK 1/2, SEK (MKK4), MKK3, or MKK6.

免疫原

synthetic phosphopeptide Ser^217/221corresponding to residues around Ser^217/221of human MEK 1/2.

应用

Anti-phospho-MAP Kinase Kinase 1/2 (MEK1/2) has a wide range of applications. The recommended working dilution for immunoblotting in serum treated NIH3T3 cells is 1:1000. For immunoprecipitation, a working dilution of 1:50 may be used. For detection by immunohistochemistry in paraffin-embedded human breast carcinoma sections, antibody dilution of 1:100 may be used. For immunocytochemistry (immunofluorescence), in HeLa cells, a working dilution of 1:1000 may be used.

外形

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02097160
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
分子量	antigen 45 kDa
species reactivity	human, mouse, rat
technique(s)	flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells. immunocytochemistry: 1:1,000 using HeLa cells immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma immunoprecipitation (IP): 1:50 western blot: 1:1,000 using serum-treated NIH-3T3 cells
UniProt登记号	P28482
运输	wet ice
储存温度	?20℃
Gene Information	human ... MAPK1(5594) mouse ... Mapk1(26413) rat ... Mapk1(116590)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable